Development of a multielectrode array (MEA) based on active recruiting of cells and formation of mechanically confined neurons

Learning and memory involve the interaction of complex networks of neurons. While much progress has been made in this area of neurobiology, a full understanding of the cellular processes underlying thought and memory, including those guiding the generation of these networks and the communication between individual neurons, remains elusive. One approach to investigate neuronal activity is to grow defined arrays of neurons on a substrate containing built-in electrodes capable of sensing the electrical signals generated by individual neurons. These multielectrode arrays (MEAs) are also capable of providing electrical stimulation in a regulated manner followed by recording of the resulting electrical activity.

 

This interdisciplinary project is a collaborative effort between Svetlana Tatic-Lucic (Electrical and Computer Engineering and Bioengineering) and Susan Perry (Bioengineering). The focus of our 2011 BDSI project is to develop a novel type of MEA, which utilizes both electrical and mechanical methods to recruit and confine neurons to specific, electrode-containing locations. Isolating individual neurons at specific locations within a predefined substrate that contains special channels for neurites will subsequently allow for predictable neurite outgrowth and ultimately the formation of a patterned neuronal network. In this project, students will aid in the design of photomasks, learn hands-on clean room fabrication techniques, assist during the fabrication process of novel MEA chips, and package the MEA devices.  Using basic cell culturing techniques, students will also culture patterned neuronal networks on their fabricated substrates, with the ultimate goal of recording electrical activity from the neurons.